ANSI NSFInternationalStandard AmericanNationalStandard NSF/ANSI 49 - 2019 Biosafety Cabinetry: Design, Construction, Performance, and Field Certification NSF NSF International, an independent, not-for-profit, nongovernmental organization, is dedicated to beingtheleadingglobalproviderofpublichealth and safety-based risk management solutions while serving the interests of all stakeholders. This Standard is subject to revision. Contact NSF to confirm this revision is current. Users of this Standard may request clarifications and interpretations,orproposerevisionsbycontacting: Chair, Joint Committee on Biosafety Cabinetry c/oNSFinternationai 789 North Dixboro Road, PO Box 130140 Ann Arbor, Michigan 48113-0140 USA Phone: (734) 769-8010 Fax: (734) 769-0109 Email: [email protected] Web: <www.nsf.org> NSF/ANSI 49 - 2019 NSF International Standard / American National Standard for Biosafety Cabinery - Biosafety Cabinetry: Design, Construction, Performance, and Field Certification Standard Developer NSF International Designated as an ANSI Standard August 1, 2019 AmericanNationalStandardsInstitute - Prepared by The NSF Joint Committee on Biosafety Cabinetry Recommended for adoption by TheNSFCouncilofPublicHealthConsultants Adopted by NSF International June 1976 Revised May 1983 Revised June 1987 Revised May 1992 Revised March 2002 Addendum November 2002 Revised February 2004 Revised September 2004 Addendum October 2004 Addendum March 2005 Revised July 2007 Revised October 2008 Revised June 2009 Revised September 2010 Revised November 2010 Revised November 2011 Revised July 2012 Revised February 2015 Revised March 2017 Revised January 2019 RevisedMarch2020 Published by NSF International PO Box 130140, Ann Arbor, Michigan 48113-0140, USA For ordering copies or for making inquiries with regard to this Standard, please reference the designation “"NSF/ANSI 49-2019" Copyright 2020 NSF International 1976 Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from NSF International. Printed in the United States of America. ii Disclaimers NSF International (NsF), in performing its functions in accordance with its objectives, does not assume or of NSF represent its professional judgment. NSF shall not be responsible to anyone for the use of or reliance consequential damages, arising out of or in connection with the use, interpretation of, or reliance upon this purpose. NsF Standards provide basic criteria to promote sanitation and protection of public health and the environment. Provisions for mechanical and electrical safety have not been included in this Standard because governmental agencies or other national standards-setting organizations provide safety requirements. Participation in NsF Standards development activities by regulatory agency representatives (federal, local. state) shall not constitute their agency's endorsement of NSF or any of its Standards. Preference is given to the use of performance criteria measurable by examination or testing in NsF Standards development when such performance criteria may reasonably be used in lieu of design, materials,orconstruction criteria. The illustrations, if provided, are intended to assist in understanding their adjacent standard requirements. the only method of fabricating such arrangements. Such partial drawings shall not be used to justify improper or incomplete design and construction. At the time of this publication, examples of programs and processes were provided for general guidance. This information is given for the convenience of users of this standard and does not constitute an organization. 1 The information contained in this Disclaimer is not part of this American National Standard (ANS) and has not been processed in accordance with ANSI's requirements for an ANS. Therefore, this Disclaimer may contain material that forconformancetotheS